LOW MOLECULAR HYALURONIC ACID PRODUCTION:

Forlle’d laboratory has its own patent for  “Manufacturing method of Low molecular Hyaluronic acid”

Human body is composed of several substances including glycoprotein, which is a bonding of protein and sugar and has high moisture level. Glycosaminoglycan called hyaluronic acid is one of the main kinds of glycoprotein. It exists in several different places in human body, and it plays an important role as the main component of the matrix inside the connective tissue.

Hyaluronic acid is one type of glucosaminoglycans, and consists of glucuronic acid and N-acetylglucosamine. It is also called heteropolysaccharide or heteroglycan. Heteropolysaccharides consists of more than two kinds of monosaccharides and it has a simple structure. The unit of repetition of polysaccharides in many cases is disaccharides consisting of amino acids and uronic acids. Some aminoacids have the sulphate group (-SO3), polysaccharides with the sulphate group is called mucopolysaccharides because they are gelatinous.

The oxidation of the sixth alcohol group of glucose into the carboxyl group makes uronic acid, and since this uronic acid is from glucose, it is called glucuronic acid.
D-glucose to D-glucuronic acid

Maltose becomes glucose with 2 molecules by hydrolysis or the action of maltase (enzyme). The addition of amino group to glucose makes glucosamine. When the amino group of glucosamine is acetylated, they become N- acetylglucosamin, the one side radical of hyaluronic acid.

In human body each organ composes its hyaluronic acid from these components and Forlle’d technology uses specially developed in its laboratory fermentation processes that allows the formation of these materials and synthesis of hyalurunic acid.

Present hyaluronic acid is used as either hyaluronic acid or hyaluronate sodium. Usually sodium hydroxide is used to make acids soluble in water, and since other ions are not available it makes the minerals ill balanced.

Forlle’d introduced two ions beside sodium, which are calcium and magnesium and they are necessary to construct a bridge with ions that have more than two values that make hyaluronic acid form a solid matrix.

Main steps in developing Forlle’d hyaluronic acid can be summarized in the following steps:

  • Malt/organic acid fermentation,
  • sterilization,
  • addition of ionized minerals.

Reducing the size of this molecule makes it an ion and very effective tool as an antioxidant, it has a scavenging effect on lipid peroxidation and free radicals, this was proven by Chunlin Ke, et al in 2011 who studied the efficiency of different sizes of Hyaluronic acid molecule as antioxidants.

Hyaluronic acid molecule has the affinity to absorb moisture up to 6000 times its weight, trap it inside the skin and by reducing the size of it into smaller molecules this number grows significantly due to the fact that each nanoparticle will absorb certain amount of moisture and spread it uniformly across the skin while in conventional HA, core sections of this molecule doesn’t access water due to ionic repulsion.

The simple chemical structure of hyaluronic acid which is the repetition of (glucoronic acid and N-acetyl glucosamine) subunits makes it a flexible molecule to work with and reducing its size doesn’t affect its activity.

If we combine all the proceeded facts about LMHA, it will become clear how this technology stabilizes to a great extent the extracellular structure, by reducing its damage (anti oxidant), stabilizing collagen and elastin, prevent dehydration, reduce external pressure, and stimulate collagen synthesis by fibroblasts through the activation of CD44 receptor.

Know more:

Advantages of Low molecular Hyaluronic acid by Forlle’d

The role of Low molecular Hyaluronic acid in Forlle’d products



LOW MOLECULAR PLATINUM PRODUCTION:

Platinum particle size is very important since it determines its activity and safety, for example Particle size of 1/8,000 ~ 1/10,000 mm forms a colloidal substance in water solution with limited physical pulverization. This kind of colloidal platinum has poor electrical and thermal conductivity plus weak absorption. Colloidal substance has low penetration and weak antioxidant activity.

Forlle’d technology have managed to reduce the size of platinum particles to 1/1million ~ 1/10 millions through a high-tech ionization process. This allows water to become closest to the electric conductivity that metal has which is very crucial to its antioxidative function (Fig. 1).

Schema for metal particle / metal colloidal particle / Metal ion particle
Fig. 1

Forlle’d ionized low molecular platinum particles interact properly with water molecules and its dispersion is very close to aqueous solution than colloidal. This allows water molecules to carry the charge of the metal particles thus enhancing penetration and antioxidant activity.

Along with its unique antioxidant properties, platinum can also stimulate skin cells activity. This phenomenon can be explained by creating the optimal temperature for cellular environment. Platinum is one of the three metals in nature able to generate infrared rays with wavelength from 4 to 14 microns so-called "rays of growth". The action of infrared light with a wavelength of about 10 microns activates all the molecules with an electrical polarity as two waves overlap and a resonance phenomenon appears. The average human body temperature is 36.5° C, if we translate this value into a wavelength, it equals 10 microns. If we translate the value of platinum wavelength (10 microns) into temperature it will be equal to 36.5° C. This temperature is optimal for the chemical processes in the body to take place which are essential for cellular activity. Activation of molecular activity leads to the improvement of cellular metabolism and function.

For the purpose of increasing the effectiveness of platinum, it is necessary to make it part of an organic compound. This was possible with Forlle’d special technology that involve fermentation processes.

Know more:

Advantages of ionized low molecular Platinum by Forlle’d

The role of ionized Platinum in Forlle’d products

 

LOW MOLECULAR CERAMIDES PRODUCTION:

Forlle’d laboratory has its own patent for ‘’Manufacturing method of dispersion of ceramide-like’s composition”

Ceramides resemble one of the main components of the epidermis abandoned in the intercellular spaces and cell membranes. Along with saturated fatty acids and cholesterol, ceramides form a water-impermeable skin barrier that prevent excessive water loss due to evaporation and maintain the epidermal layer cells intact.

Recent studies have shown that ceramides participate in variety of cellular signaling mechanisms that regulate cellular division and differentiation.

There are six types of ceramides found in human skin; Forlle’d use ceramide type III that decline with age, especially between the adolescent and adulthood period.

Including this component in skin care products is of immense importance, considering the role of the epidermal barrier in protecting the skin against infection, inflammation and dehydration; however natural ceramides have high melting points and don’t dissolve with other ingredients easily such as water, oil or ethanol. In order to solve this problem many companies use simulated ceramides as emollients for example Cholesteryl/Octyldodecyl Lauroyl Glutamate and sphingoglycolipid instead of natural ceramides. Another approach is related to emulsifying ceramides and enhancing their dispersion with water clusters by reducing the size of the molecules.

Forlle’d introduced a patented technology that allow the production of ionized low molecular weight ceramides; this technology permit the use of natural ceramides and mix them not only with creams but lotion and water solutions as well without the use of active detergents.

Know more:

Advantages of low molecular Ceramides-3 in Forlle’d products