Mass-spectrometry analysis of Forlle’d low molecular weight Hyaluronic Acid

Forlle’d Laboratory by decreasing the size of Hyaluronic acid to 2055 Da, corresponding to 5nm in size, managed to increase the activity of this molecule and implement it in skin care industry. The graphic below from Shimatzu Laboratory (Japan) proves the low molecular weight of Hyaluronic acid achieved by Forlle’d scientists.
Graphic from Shimatzu Laboratory (Japan) about low molecular weight of Hyaluronic acid achieved by Forlle’d scientists

Penetration rate and bioavailability of Forlle’d Low molecular Hyaluronic acid

The ability of Forlle’d low molecular weight hyaluronic acid to cross the skin barrier has been studied In Vitro on a skin analogue. This experiment allowed us to measure accurately the quantity of molecules able to cross the skin barrier and their penetration rate.

Experiments showed, that Forlle’d low molecular weight Hyaluronic acid has the ability to cross the skin barrier (epidermis) and has good penetration rate reaching up to 70% within the first 8 hours after topical application. Moreover, confocal microscopy showed a very good bioavailability of Forlle’d low molecular weight Hyaluronic acid in both the epidermis (keratinocytes) and the dermis (fibroblasts).
Information experiments of ability to cross the skin barrier

Product efficiency investigation

Product efficiency tests were carried in an independent laboratory in Germany. The test included assessment of erythema level, wrinkles three dimensional structure, skin hydration and elasticity.

Measurement of skin hydration was carried with Corneometer and it increased throughout the study up to 96% in comparison to control group and reached its maximum on day 57.

Wrinkles roughness was assessed with DermaTOP by capturing three dimensional structure of the investigated skin. Wrinkles were significantly reduced on day 15 after product application and remained on comparable level throughout the study. After 57 days of daily application the depth of wrinkles reduced up to 40% in comparison to day 1.

Skin firmness or elasticity were measured with Cutometer and showed an improvement of the skin after product application up to 55% on day 57.

Redness of the skin erythema was measured with Chromameter and its value was reduced up to 35% after product application in comparison to untreated area.
Tests of erythema level, skin hydration and elasticity